Academic
Publications
Screening of genetic parameters for soluble protein expression in Escherichia coli

Screening of genetic parameters for soluble protein expression in Escherichia coli,10.1016/j.pep.2010.11.016,Protein Expression and Purification,Erik

Screening of genetic parameters for soluble protein expression in Escherichia coli   (Citations: 1)
BibTex | RIS | RefWorks Download
Soluble expression of proteins in a relevant form for functional and structural investigations still often remains a challenge. Although many biochemical factors are known to affect solubility, a thorough investigation of yield-limiting factors is normally not feasible in high-throughput efforts. Here we present a screening strategy for expression of biomedically relevant proteins in Escherichia coli using a panel of six different genetic variations. These include engineered strains for rare codon supplementation, increased disulfide bond formation in the cytoplasm and novel vectors for secretion to the periplasm or culture medium. Combining these variants with expression construct truncations design, we report on parallel cloning and expression of more than 300 constructs representing 24 selected proteins; including full-length variants of human growth factors, interleukins and growth factor binding proteins. This rapid screening approach appears highly suitable for high-throughput efforts targeting either large sets of proteins or more focused investigations regarding individual high-profile targets.
Journal: Protein Expression and Purification - PROTEIN EXPRESS PURIF , vol. 77, no. 1, pp. 104-111, 2011
Cumulative Annual
View Publication
The following links allow you to view full publications. These links are maintained by other sources not affiliated with Microsoft Academic Search.
Sort by: