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Interleukin8 of Cynoglossus semilaevis is a chemoattractant with immunoregulatory property

Interleukin8 of Cynoglossus semilaevis is a chemoattractant with immunoregulatory property,10.1016/j.fsi.2011.03.023,Fish & Shellfish Immunology,Jin-s

Interleukin8 of Cynoglossus semilaevis is a chemoattractant with immunoregulatory property   (Citations: 1)
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Interleukin-8 (IL-8), or CXCL8, is a member of the CXC chemokine family that in mammals is known to mediate inflammatory response. In this study, we identified and analyzed an IL-8 orthologue, CsIL8, from half-smooth tongue sole (Cynoglossus semilaevis). The deduced amino acid sequence of CsIL8 contains 100 residues and is closely related to the lineage 1 IL-8 of a number of fish species. In silico analysis identified in CsIL8 a CXC chemokine domain that contains four conserved cysteine residues, two of which form the CXC signature motif of CXC chemokines. Expression of CsIL8 as determined by quantitative real time RT-PCR was detected in a wide range of tissues under normal physiological conditions and was upregulated by bacterial challenge and by vaccination with a subunit vaccine. Purified recombinant CsIL8 (rCsIL8) induced migration of peripheral blood leukocytes and head kidney (HK) lymphocytes and stimulated the proliferation of these cells in a dose-dependent manner. When rCsIL8 was added to the cell culture of HK lymphocytes, it upregulated the expression of interleukin-1β and CsIL8 in a time-dependent fashion. These results indicate that CsIL8 is a biologically active CXC chemokine with immunoregulatory activity and that CsIL8 is involved in pathogen-induced inflammatory response.
Journal: Fish & Shellfish Immunology - FISH SHELLFISH IMMUNOL , vol. 30, no. 6, pp. 1362-1367, 2011
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