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Effects of 50 Hz sinusoidal electromagnetic fields of different intensities on proliferation, differentiation and mineralization potentials of rat osteoblasts

Effects of 50 Hz sinusoidal electromagnetic fields of different intensities on proliferation, differentiation and mineralization potentials of rat ost

Effects of 50 Hz sinusoidal electromagnetic fields of different intensities on proliferation, differentiation and mineralization potentials of rat osteoblasts  
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Electromagnetic fields (EMFs) have been used clinically to slow down osteoporosis and promote fracture healing for many years. However, the underlying action mechanisms and optimal parameters of the EMF applications are unclear. In this study, we investigated the effects of treatment for different durations with 50Hz sinusoidal electromagnetic fields (SEMFs) at different intensities on proliferation, differentiation and mineralization potentials of rat osteoblasts. Osteoblasts isolated from neonatal rats were treated with SEMFs (50Hz at 0.9mT–4.8mT, 0.3mT interval, 30min/day up to 15days). Compared to untreated control, SEMFs inhibited osteoblast proliferation (after 3days' treatment) but increased alkaline phosphatase (ALP) activity (after treatment for 9days) from 0.9mT to 1.8mT, declined from 1.8mT until 3.0mT, and then increased again from 3.0mT to 3.6mT and decreased once again from 3.6mT to 4.8mT. Numbers of colonies stained positive for ALP after 8days and mineralized nodules stained by Alizarin red after 10days showed the same bimodal tendency as with the ALP activity, with two peaks at 1.8mT and 3.6mT. SEMFs also bimodally increased Runx-2, Col1α2 and Bmp-2 mRNA expression levels in osteoblasts at 12, 24 and 96h after exposure. The results indicated that while exposure to 50Hz SEMFs inhibits the osteoblast proliferation, it significantly promotes differentiation and mineralization potentials of osteoblasts in an intensity-dependent manner with peak activity at 1.8mT and 3.6mT.
Journal: Bone , vol. 49, no. 4, pp. 753-761, 2011
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