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Development and Preliminary Application of Monoclonal Antibody against Heavy Metal Cadmium Ion

Development and Preliminary Application of Monoclonal Antibody against Heavy Metal Cadmium Ion,10.1109/CDCIEM.2011.561,Zhang Haitang,Jiang Jinqing,Wan

Development and Preliminary Application of Monoclonal Antibody against Heavy Metal Cadmium Ion  
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Artificial hapten Cd-ITCBE was synthesized using isotrhiocyanobenzyl-EDTA(ITCBE) to chelate Cd 2+ . The isothiocyanate method was used to conjugate Cd-ITCBE to bovine serum albumin (BSA) and artificial immunogen Cd- ITCBE-BSA was obtained, the detection antigen Cd-ITCBE- OVA was obtained in the same way. Ultraviolet (UV), sodium dodecyl sulfate polyacrylamide gel electropheresis (SDS-PAGE) and inductively coupled plasma atomic emission spectroscopy (ICP-AES) were used to identify Cd-ITCBE-BSA and Cd- ITCBE-OVA. Balb/C mice were immunized with Cd-ITCBE- BSA and hybridoma lines that secreted Cd 2+ mAb were generated by cell fusion and then screened by indirect ELISA, blocking ELISA. The Cd 2+ -Kit was established with Cd 2+ mAb and its traits were tested. The Results showed that Cd-ITCBE- BSA was synthesized successfully and its concentration of BSA and Cd 2+ was 7.1 mg/mL and 191.7 μg/mL respectively. Four
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