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Dual targeting of plastid division protein FtsZ to chloroplasts and the cytoplasm

Dual targeting of plastid division protein FtsZ to chloroplasts and the cytoplasm,10.1038/sj.embor.7400238,Embo Reports,Justine Kiessling,Anja Martin,

Dual targeting of plastid division protein FtsZ to chloroplasts and the cytoplasm   (Citations: 26)
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FtsZ is a filament-forming protein that assembles into a ring at the division site of prokaryotic cells. As FtsZ and tubulin share several biochemical and structural similarities, FtsZ is regarded as the ancestor of tubulin. Chloroplasts—the descendants of endosymbiotic bacteria within plant cells—also harbour FtsZ. In contrast to eubacteria, plants have several different FtsZ isoforms. So far, these isoforms have only been implicated with filamentous structures, rings and networks, inside chloroplasts. Here, we demonstrate that a novel FtsZ isoform in the moss Physcomitrella patens is located not only in chloroplasts but also in the cytoplasm, assembling into rings in both cell compartments. These findings comprise the first report on cytosolic localization of a eukaryotic FtsZ isoform, and indicate that this protein might connect cell and organelle division at least in moss.
Journal: Embo Reports - EMBO REP , vol. 5, no. 9, pp. 889-894, 2004
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    • ...patens has been established as a plant system to investigate the evolution of stress adaptation (Frank et al. 2007; Khandelwal et al. 2010) and of signalling events (Heintz et al. 2004, 2006) in early land plants...

    Erika G. E. Langet al. Simultaneous isolation of pure and intact chloroplasts and mitochondri...

    • ...The FtsZ proteins are encoded by a small gene family, whereby some members (that have been lost during land–plant evolution) are localized in the cytoplasm and possibly interact with the cytoskeleton of the “host cell” (Kiessling et al. 2004)...

    Peter Nick. A green version of Haeckel’s rule

    • ...We tested the prediction results using the model plant Physcomitrella patens because dual targeting has been described to occur in this organism (Richter et al., 2002; Kiessling et al., 2004; Kabeya & Sato, 2005), detection of fluorescent protein fusions using transient protoplast transfection assays is a standard technique (Frank et al., 2005; Quatrano et al., 2007) and the genome sequence is available (Rensing et al., 2008)...
    • ...MitoTracker green FM (Invitrogen), mAV4–CFP and the signal peptide of FtsZ1-2 in-frame with GFP, which has been described as chloroplast localized (Kiessling et al., 2004), were used as controls...
    • ...MitoTracker green FM, mAV4–CFP (encoding no targeting signal) and the signal peptide of a plastid division protein (FtsZ1-2) in-frame with GFP (previously described as chloroplast localized (Kiessling et al., 2004)), were used as controls...

    Jan Mitschkeet al. Prediction of dual protein targeting to plant organelles

    • ...Each PpFtsZ protein has a distinct subcellular localisation, and the four PpFtsZ proteins specifically interact with each other to build higher-order structures (Kiessling et al. 2000, 2004; Gremillon et al. 2007)...
    • ...Isolation and transfection of protoplasts were carried out as described previously (Rother et al. 1994; Kiessling et al. 2004)...
    • ...PpFtsZ3 is dual-targeted, assembles into rings in the plastids as well as in the cytosol, and acts in cell and plastid division (Kiessling et al. 2004)...

    A. Martinet al. A uniquely high number of fts Z genes in the moss Physcomitrella paten...

    • ...As transient overexpression of this gene hampered cell and chloroplast division, it was suggested that this dually targeted protein may represent a molecular link between cell and organelle division in moss (Kiessling et al., 2004)...
    • ...The most obvious explanation is a disturbed interaction with PpFtsZ3, which is dually targeted to plastids and to the cytosol in P. patens (Kiessling et al., 2004), and which is interacting with PpFtsZ2-2 in wild-type moss (Gremillon et al., 2007)...
    • ...It was previously shown that expression of ftsZ3:gfp labeled plastidic as well as cytosolic rings in P. patens protoplasts, protonema cells, and leaf cells, indicating that this protein may be a molecular link between cell and chloroplast division in moss (Kiessling et al., 2004)...

    Anja Martinet al. Targeted Gene Knockouts Reveal Overlapping Functions of the Five Physc...

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