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Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

Gapped BLAST and PSI-BLAST: a new generation of protein database search programs,10.1093/nar/25.17.3389,Nucleic Acids Research,Stephen F. Altschul,Tho

Gapped BLAST and PSI-BLAST: a new generation of protein database search programs   (Citations: 25606)
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The BLAST programs are widely used tools for searching protein and DNA databases for sequence similarities. For protein comparisons, a variety of definitional, algorithmic and statistical refinements described here permits the execution time of the BLAST programs to be decreased substantially while enhancing their sensitivity to weak similarities. A new criterion for triggering the extension of word hits, combined with a new heuristic for generating gapped alignments, yields a gapped BLAST program that runs at approximately three times the speed of the original. In addition, a method is introduced for automatically combining statistically significant alignments pro- duced by BLAST into a position-specific score matrix, and searching the database using this matrix. The resulting Position-Specific Iterated BLAST (PSI- BLAST) program runs at approximately the same speed per iteration as gapped BLAST, but in many cases is much more sensitive to weak but biologically relevant sequence similarities. PSI-BLAST is used to uncover several new and interesting members of the BRCT superfamily.
Journal: Nucleic Acids Research - NAR , vol. 25, no. 17, pp. 3389-3402, 1997
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