Academic
Publications
Surface Tongue-and-groove Contours on Lens MIP Facilitate Cell-to-cell Adherence

Surface Tongue-and-groove Contours on Lens MIP Facilitate Cell-to-cell Adherence,10.1006/jmbi.2000.3920,Journal of Molecular Biology,Dimitrios Fotiadi

Surface Tongue-and-groove Contours on Lens MIP Facilitate Cell-to-cell Adherence   (Citations: 68)
BibTex | RIS | RefWorks Download
The lens major intrinsic protein (MIP, AQP0) is known to function as a water and solute channel. However, MIP has also been reported to occur in close membrane contacts between lens fiber cells, indicating that it has adhesive properties in addition to its channel function. Using atomic force and cryo-electron microscopy we document that crystalline sheets reconstituted from purified ovine lens MIP mostly consisted of two layers. MIP lattices in the apposing membranes were in precise register, and determination of the membrane sidedness demonstrated that MIP molecules bound to each other via their extracellular surfaces. The surface structure of the latter was resolved to 0.61nm and revealed two protruding domains providing a tight “tongue-and-groove” fit between apposing MIP molecules. Cryo-electron crystallography produced a projection map at 0.69nm resolution with a mirror symmetry axis at 45° to the lattice which was consistent with the double-layered nature of the reconstituted sheets. These data strongly suggest an adhesive function of MIP, and strengthen the view that MIP serves dual roles in the lens.
Journal: Journal of Molecular Biology - J MOL BIOL , vol. 300, no. 4, pp. 779-789, 2000
Cumulative Annual
View Publication
The following links allow you to view full publications. These links are maintained by other sources not affiliated with Microsoft Academic Search.
Sort by: