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Antibody Responses in Reindeer (Rangifer tarandus) Infected with Mycobacterium bovis

Antibody Responses in Reindeer (Rangifer tarandus) Infected with Mycobacterium bovis,10.1128/CDLI.12.6.727-735.2005,Clinical and Vaccine Immunology,W.

Antibody Responses in Reindeer (Rangifer tarandus) Infected with Mycobacterium bovis   (Citations: 13)
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Despite having a very low incidence of disease, reindeer (Rangifer tarandus) are subject to tuberculosis (TB) testing requirements for interstate shipment and herd accreditation in the United States. Improved TB tests are desperately needed, as many reindeer are falsely classified as reactors by current testing procedures. Sera collected sequentially from 11 (experimentally) Mycobacterium bovis-infected reindeer and 4 noninfected reindeer were evaluated by enzyme-linked immunosorbent assay (ELISA), immunoblotting, and multiantigen print immunoassay (MAPIA) for antibody specific to M. bovis antigens. Specific antibody was detected as early as 4 weeks after challenge with M. bovis. By MAPIA, sera were tested with 12 native and recombinant antigens, which were used to coat nitrocellulose. All M. bovis-infected reindeer developed responses to MPB83 and a fusion protein, Acr1/MPB83, and 9/11 had responses to MPB70. Other antigens less commonly recognized included MPB59, ESAT-6, and CFP10. Administration of purified protein derivatives for skin testing boosted serum antibody responses, as detected by each of the assays. Of the noninfected reindeer, 2/4 had responses that were detectable immediately following skin testing, which correlated with pathological findings (i.e., presence of granulomatous lesions yet the absence of acid-fast bacteria). The levels of specific antibody produced by infected reindeer appeared to be associated with disease progression but not with cell-mediated immunity. These findings indicate that M. bovis infection of reindeer elicits an antibody response to multiple antigens that can be boosted by skin testing. Serological tests using carefully selected specific antigens have potential for early detection of infections in reindeer.
Journal: Clinical and Vaccine Immunology - CLIN VACCINE IMMUNOL , vol. 12, no. 6, pp. 727-735, 2005
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    • ...The WCS was prepared as described previously (10)...
    • ...Serum antibody responses to mycobacterial antigens were boosted by intradermal injection of PPDs for skin testing (Fig. 1 and 2), as demonstrated previously with other species (5, 7, 10)...

    Dawn M. Zimmermanet al. Safety and Immunogenicity of the Mycobacterium tuberculosis  lysA  pan...

    • ...The antigens used for immunoblot analysis included a whole-cell sonicate (WCS) of M. bovis strain 95-1315 prepared as previously described (44) and recombinant MPB83 protein (Veterinary Sciences Division, Agri-Food and Biosciences Institute, Belfast, Northern Ireland)...
    • ...This surface-associated antigen of M. bovis has also been shown to be serodominant for whitetailed deer (45), reindeer (44), and Eurasian badgers (11)...
    • ...Interestingly, the high rate of seroreactivity to ESAT-6/CFP10 reported here contrasts with recent studies of white-tailed deer (45) and reindeer (44) that showed much lower levels of seroreactivity (30%) for the same hybrid protein...
    • ...It was surprising and important to note that no tuberculous animals recognized the 16-kDa Acr1 protein, since previous studies with cattle, white-tailed deer, and reindeer have all demonstrated seroreactivity (27, 44, 45)...
    • ...This phenomenon has been similarly reported in studies of various other species, including white-tailed deer and reindeer (44, 45)...

    Noel P. Harringtonet al. Antibody Responses of Cervids (Cervus elaphus) following Experimental ...

    • ...More recently, this method was adopted to determine antigen recognition patterns in cattle, white-tailed deer, reindeer, and European badgers infected with M. bovis (9, 18, 33, 34)...
    • ...However, in other host species, including humans, badgers, cattle, white-tailed deer, and reindeer, this antigen is not the most important antigenic target for serum antibodies (9, 15, 16, 18, 33, 34)...

    Konstantin P. Lyashchenkoet al. Tuberculosis in Elephants: Antibody Responses to Defined Antigens of M...

    • ...Previous research has shown that monitoring of the kinetics of production of serum antibodies to multiple mycobacterial antigens is useful for the characterization of disease progression and the efficacy of disease treatment and as a tool for the diagnosis of M. bovis or M. tuberculosis infection in several species (11, 12, 21, 23, 24, 25)...
    • ...However, when this antigen was combined with the 16-kDa protein (also known as Acr1), it was the most readily detected antigen by MAPIA in this study and was one of the most readily detected antigens in other studies as well (6, 13, 23, 24)...

    P. Nolet al. Humoral Immune Responses of White-Tailed Deer (Odocoileus virginianus ...

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