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DNA sequence analysis of the genetic environment of various blaCTX-M genes

DNA sequence analysis of the genetic environment of various blaCTX-M genes,C. Eckert,V. Gautier,G. Arlet

DNA sequence analysis of the genetic environment of various blaCTX-M genes   (Citations: 43)
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Published in 2006.
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    • ...Moreover, some of the blaCTX-M genes are part of transposons or are included in integron structures [11, 20]...

    Karim Ben Slamaet al. Diversity of Genetic Lineages Among CTX-M-15 and CTX-M-14 Producing Es...

    • ...The blaCTX-M-15 gene of the outbreak isolates from center Z1 was located in the structure (Fig. 1) originally identified in the IncFII plasmid pC15-1a from Canada (8) and later in other studies (14, 24, 27)...
    • ...The module with blaCTX-M-3a in isolate 100 from center S was identical to the mobile element of pCTX-M3-type plasmids in Poland (3, 17) and seen also in France (14) (Fig. 1). FIG. 1. Schematic representation of the genetic structures identified in the study: the ISEcp1–blaCTX-M-15 (top) and ISEcp1–blaCTX-M-3a (middle) transposition modules and the IS26–blaCTX-M-3a–IS26 element (bottom)...
    • ...It is difficult to judge whether blaCTX-M-3a was originally mobilized by IS26 or, e.g., by ISEcp1 followed by secondary IS26 insertions like those in some other blaCTX-M-3a or blaCTX-M-15 genes (14, 40)...

    E. Literackaet al. blaCTX-M Genes in Escherichia coli Strains from Croatian Hospitals Are...

    • ...The presence of an ISEcp1 element upstream of the blaCTX-M-15 gene and an orf477 element downstream of the blaCTX-M-15 gene has been described previously (10), and ISEcp1 may contribute to the mobilization and high-level expression of the bla gene...
    • ...Interestingly, the CTX-M-15-like enzyme has been identified in an E. coli clinical isolate in China, and the blaCTX-M-64 gene as well as the blaCTX-M-15-like gene has been located 45 bp downstream from ISEcp1, while the spacer region between ISEcp1 and blaCTX-M-15 is generally 48 bp in length (10, 17, 24)...

    Yukiko Naganoet al. Novel Chimeric  Lactamase CTX-M-64, a Hybrid of CTX-M-15Like and CTX-M...

    • ...The genetic environment of blaCTX-M genes was also tested by PCR and by sequencing with previously reported primers (10, 15, 22)...

    Patricia Poetaet al. Seagulls of the Berlengas Natural Reserve of Portugal as Carriers of F...

    • ...Finally, the sequencing of the fragments containing TEM-1 from E. coli 2138 and E. cloacae 7506 revealed the presence of two genes coding for a resolvase, TnpA, and its regulator, TnpR, present upstream from blaTEM-1 in both strains (Fig. 2C), as previously described for other blaTEM-1-containing plasmids of clinical origin (19)...

    Stephanie Petrellaet al. Genetic and Structural Insights into the Dissemination Potential of th...

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