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The TOM Core Complex: The General Protein Import Pore of the Outer Membrane of Mitochondria

The TOM Core Complex: The General Protein Import Pore of the Outer Membrane of Mitochondria,10.1083/jcb.147.5.959,Journal of Cell Biology,Uwe Ahting,C

The TOM Core Complex: The General Protein Import Pore of the Outer Membrane of Mitochondria   (Citations: 69)
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Translocation of nuclear-encoded prepro- teins across the outer membrane of mitochondria is me- diated by the multicomponent transmembrane TOM complex. We have isolated the TOM core complex of Neurospora crassa by removing the receptors Tom70 and Tom20 from the isolated TOM holo complex by treatment with the detergent dodecyl maltoside. It con- sists of Tom40, Tom22, and the small Tom components, Tom6 and Tom7. This core complex was also purified di- rectly from mitochondria after solubilization with dodecyl maltoside. The TOM core complex has the characteristics of the general insertion pore; it contains high-conductance channels and binds preprotein in a targeting sequence-dependent manner. It forms a dou- ble ring structure that, in contrast to the holo complex, lacks the third density seen in the latter particles. Three-dimensional reconstruction by electron tomogra- phy exhibits two open pores traversing the complex with a diameter of z 2.1 nm and a height of z 7 nm. Tom40 is the key structural element of the TOM core complex.
Journal: Journal of Cell Biology - J CELL BIOL , vol. 147, no. 5, pp. 959-968, 1999
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    • ... In addition to single-particle electron microscopic analysis of purified translocase complexe...

    Oliver Schmidtet al. Mitochondrial protein import: from proteomics to functional mechanisms

    • ...Overall, N. crassa has been vastly used in mitochondria research, such as studies of the protein import machinery (2, 43, 75)...
    • ...The mitochondrial import receptor Tom40 (Fig. 2, spot 42) was also identified as a subunit of the TOM holo or core complex (2, 63, 75, 76, 82) and found to comigrate with monomeric complex IV and heterooligomeric NAD-dependent isocitrate dehydrogenase (Fig. 2, spots 40 and 41)...
    • ...Tom40 is the main component of the TOM complex and essential for viability of N. crassa (2, 82)...

    Isabel Marqueset al. Supramolecular Organization of the Respiratory Chain in Neurospora cra...

    • ... to a variety of other protein translocating channels, including the PapC outer membrane usher involved in the assembly of E. coli P pili, the Sec complex responsible for transport of proteins across the bacterial inner membrane and the eukaryotic endoplasmic reticulum, the TIM22 protein insertion complex in the mitochondrial inner membrane, and the TOM protein import complex in the mitochondrial outer membrane, among others (1, 2, 4, 20, ...

    Huilin Liet al. Structure of the Haemophilus influenzae HMW1B Translocator Protein: Ev...

    • ...Interestingly, a similar twin-pore structure has been identified for the unrelated TOM protein translocase of the mitochondrial OM (1)...

    Maria Kostakiotiet al. Mechanisms of Protein Export across the Bacterial Outer Membrane

    • ...This complex is composed of at least seven different subunits (Fig. 1). Tom20 and 70 are the primary receptors, whereas the subunits Tom40, 22, 7, 6, and 5 form the stable TOM core complex (Fig. 1; Dekker et al., 1998; Künkele et al., 1998; Ahting et al., 1999)...
    • ...The TOM complex was indeed suggested to harbor six to eight molecules of Tom40 (Dekker et al., 1998; Künkele et al., 1998), and electron microscopic visualization of the TOM complex revealed the presence of two to three pores (Künkele et al., 1998; Ahting et al., 1999; Model et al., 2002)...

    Doron Rapaport. How does the TOM complex mediate insertion of precursor proteins into ...

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