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Heparan Sulfate Proteoglycans Function as Receptors for Fibroblast Growth Factor2 Activation of Extracellular Signal-Regulated Kinases 1 and 2

Heparan Sulfate Proteoglycans Function as Receptors for Fibroblast Growth Factor2 Activation of Extracellular Signal-Regulated Kinases 1 and 2,Ceres C

Heparan Sulfate Proteoglycans Function as Receptors for Fibroblast Growth Factor2 Activation of Extracellular Signal-Regulated Kinases 1 and 2   (Citations: 17)
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Abstract—Fibroblast growth factor-2 (FGF2) activates the extracellular signal–regulated kinases 1 and 2 (ERK1/2) through its specific receptors. Interaction of FGF2 with cell-surface heparan sulfate proteoglycans has also been suggested to induce intracellular signals. Thus, we investigated whether FGF2 can stimulate ERK1/2 activation through heparan sulfate proteoglycans using mechanisms,that do not depend on receptor activation in vascular smooth muscle cells. The activation of FGF receptors was inhibited by treating cells with 5-deoxy-5methyl-thioadenosine and by expressing truncated dominant-negative FGF receptors. In both cases, FGF2 was able to stimulate the phosphorylation of ERK1/2 despite the absence of detectable FGF receptor tyrosine kinase activity. The FGF2 activation of ERK1/2 in the absence of receptor activity was completely dependent on heparan sulfate, because this activity was abolished by heparinase III digestion of the cells. In contrast, heparinase III treatment of control cells, with functional FGF receptors, showed only
Published in 2010.
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