Top publications in molecular biology 1–100 of 367,162 results
Publications Citations  
1
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs (1997) 25606
2
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice (1994) 24556
3
Basic local alignment search tool (1990) 21081
4
The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools (1997) 11581
5
A simple salting out procedure for extracting DNA from human nucleated cells (1988) 7037
6
The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools (1997) 5888
7
A comprehensive set of sequence analysis programs for the VAX (1984) 5886
8
AFLP: a new technique for DNA fingerprinting (1995) 4950
9
A rapid alkaline extraction procedure for screening recombinant plasmid DNA (1979) 4641
10
DNA polymorphisms amplified by arbitrary primers are useful as genetic markers (1990) 4461
11
Tissue sulfhydryl groups (1959) 4254
12
A new mathematical model for relative quantification in real-time RT-PCR (2001) 3622
13
Rapid isolation of high molecular weight plant DNA (1980) 2822
14
Mfold web server for nucleic acid folding and hybridization prediction (2003) 2734
15
MUSCLE: multiple sequence alignment with high accuracy and high throughput (2004) 2734
16
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei (1983) 2224
17
Fingerprinting genomes using PCR with arbitrary primers (1990) 2187
18
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes (2002) 2095
19
CDK inhibitors: positive and negative regulators of G1-phase progression (1999) 2043
20
Bioconductor: open software development for computational biology and bioinformatics (2004) 1955
21
ARB: a software environment for sequence data (2004) 1904
22
The Pfam protein families database (2004) 1777
23
Linear Models and Empirical Bayes Methods for Assessing Differential Expression in Microarray Experiments (2004) 1743
24
Multiple sequence alignment with hierarchical clustering (1988) 1742
25
The Pfam Protein Families Database (2001) 1732
26
Normalization for cDNA microarray data: a robust composite method addressing single and multiple slide systematic variation (2002) 1635
27
KEGG: Kyoto Encyclopedia of Genes and Genomes (2000) 1624
28
An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs (1987) 1612
29
Rapid detection of octamer binding proteins with ‘mini extracts’, prepared from a small number of cells (1989) 1610
30
Cellular survival: a play in three Akts (1999) 1574
31
Lipid rafts and signal transduction (2000) 1563
32
A new mathematical model for relative quantification in real-time RT-PCR (2001) 1528
33
Summaries of Affymetrix GeneChip probe level data (2003) 1470
34
The Pfam Protein Families Database (2002) 1464
35
SWISS-MODEL: an automated protein homology-modeling server (2003) 1447
36
tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence (1997) 1426
37
Multiple sequence alignment with the Clustal series of programs (2003) 1417
38
MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0 (2007) 1408
39
Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae (1998) 1375
40
Inhibitors of mammalian G1 cyclin-dependent kinases (1995) 1349
41
BCL2 family members and the mitochondria in apoptosis (1999) 1345
42
Relative expression software tool (REST(C)) for group-wise comparison and statistical analysis of relative expression results in real-time PCR (2002) 1344
43
Matind and matinspector - new fast and versatile tools for detection of consensus matches in nucleotide sequence data (1995) 1341
44
The SWISS-PROT protein knowledgebase and its supplement TrEMBL in 2003 (2003) 1328
45
GenBank (2003) 1324
46
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian (1983) 1313
47
DAVID: Database for Annotation, Visualization, and Integrated Discovery (2003) 1298
48
Two CD95 (APO1/Fas) signaling pathways (1998) 1289
49
Multiple sequence alignment with Clustal X (1998) 1281
50
Quantitative analysis of dose-effect relationships: the combined effects of multiple drugs or enzyme inhibitors (1984) 1246
51
DNA methylation patterns and epigenetic memory (2002) 1241
52
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter (1984) 1217
53
A new method for predicting signal sequence cleavage sites (1986) 1209
54
DNA polymorphism amplified by arbitrary primers are useful as genetic markers (1990) 1137
55
A genome-wide transcriptional analysis of the mitotic cell cycle (1998) 1135
56
Improved method for high efficiency transformation of intact yeast cells (1992) 1135
57
Tandem repeats finder: a program to analyze DNA sequences (1999) 1119
58
‘Touchdown’ PCR to circumvent spurious priming during gene amplification (1991) 1115
59
Untangling the ErbB signalling network (2001) 1111
60
A catalogue of splice junction sequences (1982) 1100
61
AFLP: a new technique for DNA fingerprinting (1986) 1096
62
Single cell gel/comet assay: Guidelines for in vitro and in vivo genetic toxicology testing (2000) 1092
63
The SWISS-PROT protein sequence database and its supplement TrEMBL in 2000 (2000) 1080
64
Caspases: killer proteases (1997) 1080
65
Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy (1989) 1079
66
RNA interference is mediated by 21- and 22-nucleotide RNAs (2001) 1062
67
Photoperoxidation in isolated chloroplasts I. Kinetics and stoichiometry of fatty acid peroxidation (1968) 1052
68
Pfam: clans, web tools and services (2006) 1041
69
IAP family proteins---suppressors of apoptosis (1999) 1034
70
Gene Expression Omnibus: NCBI gene expression and hybridization array data repository (2002) 1032
71
The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes (1963) 1013
72
Sequence logos: a new way to display consensus sequences (1990) 987
73
New heterologous modules for classical or PCR-based gene disruptions inSaccharomyces cerevisiae (1994) 978
74
RNA splice junctions of different classes of eukaryotes: sequence statistics and functional implications in gene expression (1987) 960
75
Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information (1981) 953
76
A rapid micrqpreparation technique for extraction of DNA-binding proteins from limiting numbers of mammalian cells (1991) 951
77
Distribution of repetitive DNA sequences in eubacteria and application to finerpriting of bacterial enomes (1991) 946
78
PSORT: a program for detecting sorting signals in proteins and predicting their subcellular localization (1999) 946
79
p53: puzzle and paradigm (1996) 945
80
The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid (1961) 935
81
Wnt signaling: a common theme in animal development (1997) 933
82
Rho GTPases and signaling networks (1997) 932
83
RASMOL: biomolecular graphics for all (1995) 930
84
LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing (2000) 916
85
Free radicals and antioxidants in normal physiological functions and human disease (2007) 914
86
High efficiency transformation of E. coli by high voltage electroporation (1988) 907
87
From genomics to chemical genomics: new developments in KEGG (2006) 907
88
The Pfam protein families database (2008) 905
89
The mitochondrial permeability transition (1995) 899
90
The Pfam Protein Families Database (2000) 883
91
Determination of DNA base composition by reversed-phase high-performance liquid chromatography (1984) 879
92
Stable Isotope Labeling by Amino Acids in Cell Culture, SILAC, as a Simple and Accurate Approach to Expression Proteomics (2002) 875
93
Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family (1985) 873
94
The regulation of E2F by pRB-family proteins (1998) 862
95
The codon adaptation index-a measure of directional synonymous codon usage bias, and its potential applications (1987) 850
96
Application of Phylogenetic Networks in Evolutionary Studies (2005) 830
97
Database resources of the National Center for Biotechnology Information (2005) 828
98
Binary Agrobacterium vectors for plant transformation (1984) 826
99
osteoprotegerin-deficient mice develop early onset osteoporosis and arterial calcification (1998) 819
100
Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the gene (1985) 817