Top publications in chemistry 1–100 of 4,418,906 results
Publications Citations  
1
A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding (1976) 58610
2
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs (1997) 25606
3
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction (1987) 24908
4
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice (1994) 24556
5
Basic local alignment search tool (1990) 21081
6
The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools (1997) 11581
7
Detection of specific sequences among DNA fragments separated by gel electrophoresis (1975) 9304
8
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity (1983) 9286
9
Colorimetric Method for Determination of Sugars and Related Substances (1956) 8559
10
Macromolecules (1939) 8555
11
A short history of SHELX (2008) 8187
12
A simple salting out procedure for extracting DNA from human nucleated cells (1988) 7037
13
[20] Processing of X-ray diffraction data collected in oscillation mode (1997) 6762
14
Measurement of protein using bicinchoninic acid (1985) 6567
15
A simple method for displaying the hydropathic character of a protein (1982) 6214
16
Crystallography & NMR System: A New Software Suite for Macromolecular Structure Determination (1998) 6175
17
PROCHECK: a program to check the stereochemical quality of protein structures (1993) 5930
18
MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures (1991) 5904
19
The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools (1997) 5888
20
A comprehensive set of sequence analysis programs for the VAX (1984) 5886
21
Physical Chemistry (1930) 5750
22
Intermolecular and Surface Forces (1992) 5671
23
Improved methods for building protein models in electron density maps and the location of errors in these models (1991) 5623
24
A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding (1976) 5429
25
Free Radicals in Biology and Medicine (1999) 5235
26
A modified single solution method for the determination of phosphate in natural waters (1962) 5154
27
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease (1979) 5021
28
Density-functional thermochemistry. III. The role of exact exchange (1992) 4958
29
AFLP: a new technique for DNA fingerprinting (1995) 4950
30
Gene ontology: tool for the unification of biology. The Gene Ontology Consortium (2000) 4930
31
The properties of gases and liquids (1987) 4892
32
Basic Local Alignment Search Tool (1990) 4678
33
Principles of fluorescence spectroscopy (1999) 4668
34
Electrochemical Methods: Fundamentals and Applications (1980) 4654
35
A rapid alkaline extraction procedure for screening recombinant plasmid DNA (1979) 4641
36
Principles of Polymer Chemistry 4631
37
Helical microtubules of graphitic carbon (1991) 4606
38
DNA polymorphisms amplified by arbitrary primers are useful as genetic markers (1990) 4461
39
Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction (1979) 4425
40
Handbook of Chemistry and Physics (1981) 4328
41
Tissue sulfhydryl groups (1959) 4254
42
Refinement of Macromolecular Structures by the Maximum-Likelihood Method (1997) 4096
43
Coot: model-building tools for molecular graphics (2004) 4053
44
Organic Chemistry (1927) 3964
45
Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar (1959) 3914
46
DENSITY-FUNCTIONAL THEORY OF ATOMS AND MOLECULES (1990) 3900
47
DISC ELECTROPHORESIS - II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS (2006) 3827
48
The Nature of the Chemical Bond (1960) 3722
49
Studies on transformation of Escherichia coli with plasmids (1983) 3715
50
Scaling concepts in polymer physics (1979) 3714
51
A new mathematical model for relative quantification in real-time RT-PCR (2001) 3622
52
CHARMM: A program for macromolecular energy, minimization, and dynamics calculations (1983) 3618
53
Viscoelastic Properties of Polymers (1980) 3557
54
Revised effective ionic radii and systematic studies of interatomic distances in halides and chalcogenides (1976) 3342
55
CRC Handbook of Chemistry and Physics (1998) 3324
56
Analysis of nitrate, nitrite, and [N]nitrate in biological fluids (1982) 3281
57
Purification of Laboratory Chemicals (1988) 3271
58
Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa (1987) 3247
59
Molecular theory of gases and liquids (1954) 3230
60
A procedure for the isolation of deoxyribonucleic acid from micro-organisms† (1961) 3222
61
Enzymes (1952) 3157
62
Advanced Organic Chemistry (1958) 3131
63
Gaussian 94 Revision E (1995) 3131
64
Identification of common molecular subsequences (1981) 3041
65
Ab Initio Molecular Orbital Theory (1986) 3031
66
Polymer Handbook (1972) 3016
67
ORTEP-3 for Windows - a version of ORTEP-III with a Graphical User Interface (GUI) (1997) 2946
68
Infrared Spectra of Inorganic and Coordination Compounds (1970) 2873
69
Sequence and organization of the human mitochondrial genome (1981) 2873
70
Physical Chemistry of Surfaces (1982) 2833
71
Comparison of simple potential functions for simulating liquid water (1983) 2833
72
Molecular dynamics with coupling to an external bath (1984) 2824
73
Rapid isolation of high molecular weight plant DNA (1980) 2822
74
Advanced Inorganic Chemistry (1988) 2786
75
Spectrometric Identification of Organic Compounds (1981) 2757
76
Mfold web server for nucleic acid folding and hybridization prediction (2003) 2734
77
MUSCLE: multiple sequence alignment with high accuracy and high throughput (2004) 2734
78
NMRPipe: A multidimensional spectral processing system based on UNIX pipes (1995) 2654
79
SCOP: A Structural Classification of Proteins Database for the Investigation of Sequences and Structures (1995) 2593
80
The protein data bank: A computer-based archival file for macromolecular structures (1977) 2478
81
The PyMOL Molecular Graphics System (2002) 2412
82
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I*1 (1977) 2406
83
A simplification of the protein assay method of Lowry et al. which is more generally applicable (1977) 2402
84
Sequential extraction procedure for the speciation of particulate trace metals (1979) 2374
85
Liquid Crystals (1912) 2356
86
International Tables for X-Ray Crystallography (1953) 2352
87
Nmr of proteins and nucleic acids (1986) 2336
88
THE UBIQUITIN SYSTEM (1998) 2321
89
SWISS-MODEL and the Swiss-Pdb Viewer: An environment for comparative protein modeling (1997) 2298
90
A general method applicable to the search for similarities in the amino acid sequence of two proteins (1970) 2295
91
LIGAND: A versatile computerized approach for characterization of ligand-binding systems (1980) 2271
92
Comparative Protein Modelling by Satisfaction of Spatial Restraints (1993) 2270
93
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei (1983) 2224
94
Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels*1 (1971) 2207
95
Chemical Reaction Engineering (1999) 2196
96
Fingerprinting genomes using PCR with arbitrary primers (1990) 2187
97
WinGX suite for small-molecule single-crystal crystallography (1999) 2171
98
Numerical integration of the Cartesian equations of motion of a system with constraints: molecular dynamics of n-alkanes (1977) 2136
99
Protein folding and association: Insights from the interfacial and thermodynamic properties of hydrocarbons (1991) 2116
100
Improved Prediction of Signal Peptides: SignalP 3.0 (2004) 2104